Turkish Journal of Gastroenterology
Original Article

A Chemically Induced Experimental Colitis Model with a Simple Combination of Acetic Acid and Trinitrobenzene Sulphonic Acid


Department of Medical Biochemistry, Çanakkale Onsekiz Mart University Faculty of Medicine, Çanakkale, Turkey


Department of Medical Biochemistry, Abant İzzet Baysal University Faculty of Medicine, Bolu, Turkey


Department of General Surgery, Abant İzzet Baysal University Faculty of Medicine, Bolu, Turkey


Department of Pathology, Lokman Hekim University Faculty of Medicine, Ankara, Turkey

Turk J Gastroenterol 2023; 34: 196-202
DOI: 10.5152/tjg.2022.22174
Read: 630 Downloads: 164 Published: 14 March 2023

Background: It was aimed to induce a new experimental colitis model by using acetic acid and trinitrobenzene sulphonic acid together and to investigate the severity of inflammation biochemically and histopathologically in comparison with other models.

Methods: Fifty-six Wistar albino male rats were randomly divided into 4 groups as control, acetic acid, trinitrobenzene sulphonic acid, and combined groups, and the animals were sacrificed following the induction of colitis on the third day and on the seventh day. The serum amyloid A and myeloperoxidase were tested in plasma samples, and the tumor necrosis factor-alpha, interleukin 33, and ST2 were assayed in colon tissue samples with enzyme-linked immunosorbent assay in addition to histopathological examination.

Results: There were statistically significant differences between the combined and the control groups both on the third day and on the seventh day in all parameters. There was no difference between the acetic acid group on the seventh day and the control groups in biochemical parameters.

Conclusions: The acetic acid model forms acute colitis. The combined model is found to be more successful in forming inflammation when compared to other models.

Cite this article as: Cinpolat HY, Buğdaycı G, Şengül N, Astarcı HM. A chemically induced experimental colitis model with a simple combination of acetic acid and trinitrobenzene sulphonic acid. Turk J Gastroenterol. 2023;34(3):196-202.

EISSN 2148-5607