Basic & Translational - Original Article

Determination of B- and T- cell epitopes for Helicobacter pylori cagPAI: An in silico approach

Volume 31 · Issue 10 Publish Date: October 15, 2020
Sevgi Kalkanlı Taş Duygu Kırkık Kübra Öztürk Alpaslan Tanoğlu
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Turkish Journal of Gastroenterology

Volume 31 · Issue 10

Pages: 713-720

History

Published Online: October 15, 2020

Copyright © 2020 Sevgi Kalkanlı Taş, Duygu Kırkık, Kübra Öztürk, Alpaslan Tanoğlu

Sevgi Kalkanlı Taş
Department of Immunology, Saglik Bilimleri University School of Medicine, Istanbul, Turkey
Duygu Kırkık
Department of Medical Biology, Saglik Bilimleri University School of Medicine, Istanbul, Turkey
Kübra Öztürk
Department of Medical Biology, Saglik Bilimleri University School of Medicine, Istanbul, Turkey
Alpaslan Tanoğlu
Department of Gastroenterology, Sultan Abdulhamit Training and Research Hospital, İstanbul, Turkey
Kalkanlı Taş, S., Kırkık, D., Öztürk, K., & Tanoğlu, A. (2020). Determination of B- and T- cell epitopes for Helicobacter pylori cagPAI: An in silico approach. Turkish Journal of Gastroenterology, 31(10), 713–720. https://doi.org/10.5152/tjg.2020.19154
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Abstract

Background/Aims: Helicobacter pylori is classified as a gram-negative bacteria and can cause significant diseases, including gastric cancer, mucosa-associated lymphoid tumor, peptic ulcer, and chronic gastritis. Recent studies have shown that some autoimmune diseases are also associated with H. pylori. In the past decades, polymorphisms of certain genes of H. pylori, mechanisms and strains of H. pylori, and new therapeutic approaches have continued to be defined. Bioinformatic tools continue to be used in drug design and vaccine design. This study aimed to investigate the cag pathogenicity island (cagPAI) of H. pylori using an in silico approach, which could contribute to vaccine studies. Materials and Methods: The pathogenicity island of H. pylori was obtained from GenBank and analyzed with ClustalW software. Structures of cag Virb11 (Hp0525) and an inhibitory protein (Hp1451) were obtained, and codon optimization and secondary and tertiary structure prediction for the cagPAI of H. pylori were analyzed using Garnier-Osguthorpe-Rabson IV secondary structure prediction method and self-optimized prediction method with alignment software. The BcePred prediction server was used to distinguish linear B-cell epitopes, and prediction of T-cell was obtained with NetCTL and MHCPred. Results: According to the physicochemical parameters, the cagPAI of H. pylori was analyzed and found to be stable, and 2 B-cell epitopes of cagPAI of H. pylori and 2 T-cell epitopes of cagPAI were found in this study. Conclusion: B- and T-cell epitopes that we have identified can induce both humoral and cellular immune responses. Thus, these epitopes have a potential for vaccine studies. Consequently, this in silico analysis should be combined with other pieces of evidence, including experimental data, to assign function.

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Article Info
Published In
Journal Turkish Journal of Gastroenterology
Volume / Issue Volume 31 · Issue 10
Pages 713-720
DOI 10.5152/tjg.2020.19154
History
Published Online October 15, 2020
Copyright
Copyright (c) 2020 Sevgi Kalkanlı Taş, Duygu Kırkık, Kübra Öztürk, Alpaslan Tanoğlu
Affiliations
Sevgi Kalkanlı Taş
Department of Immunology, Saglik Bilimleri University School of Medicine, Istanbul, Turkey
Duygu Kırkık
Department of Medical Biology, Saglik Bilimleri University School of Medicine, Istanbul, Turkey
Kübra Öztürk
Department of Medical Biology, Saglik Bilimleri University School of Medicine, Istanbul, Turkey
Alpaslan Tanoğlu
Department of Gastroenterology, Sultan Abdulhamit Training and Research Hospital, İstanbul, Turkey
Cite this Article
Kalkanlı Taş, S., Kırkık, D., Öztürk, K., & Tanoğlu, A. (2020). Determination of B- and T- cell epitopes for Helicobacter pylori cagPAI: An in silico approach. Turkish Journal of Gastroenterology, 31(10), 713–720. https://doi.org/10.5152/tjg.2020.19154
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