Turkish Journal of Gastroenterology
Basic & Translational - Original Article

Up-regulation of miR-155 contributes to TNF-mediated hepatocyte apoptosis in acute liver failure

1.

Department of Gastroenterology, Wuxi People’s Hospital Affiliated to Nanjing Medical University, Wuxi, Jiangsu, China

2.

Health Service Center of Lihu Community, Wuxi Binhu District, Wuxi, Jiangsu, China

Turk J Gastroenterol 2019; 30: 475-484
DOI: 10.5152/tjg.2019.18159
Read: 146 Downloads: 6 Published: 25 July 2019

Abstract

 

Background/Aims: Acute liver failure (ALF) is due to severe immune response, resulting in massive apoptosis/necrosis of hepatocytes. The precise mechanism has not been explored yet.

 

Materials and Methods: The mouse with ALF model was induced by D-GalN/LPS; the hepatic miRNAs expression profile was evaluated by miRNA microarray and verified by RT-PCR. During the ALF in mice, the miR-155 expression was detected in the liver as well as in spleen. Then the correlation between miR-155 and inflammatory cytokines was evaluated. Furthermore, the miR-155 expression in activated Raw264.7 cells and apoptotic hepatocytes was also studied. Finally, the regulatory roles of miR-155 in TNF expression of apoptotic hepatocytes were shown.

 

Results: It was shown that miRNAs changed in the mice with ALF relating to hepatocytes apoptosis/necrosis; the selected miRNAs were confirmed with RT-PCR. miR-155 was up-regulated, but miR-698, -720, and -329 were down-regulated. Moreover, hepatic miR-155 was up-regulated at all-time points in the liver, but only at 7 h in spleen of mice with ALF. A significant correlation was observed between hepatic miR-155 and TNF/IL-6 in mice with ALF, which was supported by the findings in vitro showing up-regulated miR-155 in Raw264.7 cells and Hepa1-6 cells under LPS or D-GalN+TNF induction, respectively. Moreover, a correlation was observed between miR155 and TNF levels in vivo and in vitro.

 

Conclusion: These data demonstrate that miR-155 regulates TNF-mediated hepatocyte apoptosis in ALF, which provides some useful information in both basic and clinical researches.

 

Cite this article as: Zhang G, Yan C, Chen D, et al. Up-regulation of miR-155 contributes to TNF-mediated hepatocyte apoptosis in acute liver failure. Turk J Gastroenterol 2019; 30(5): 475-84.

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