Turkish Journal of Gastroenterology
Basic & Translational - Original Article

Methylprednisolone prevents bacterial translocation in thioacetamide-induced liver failure in rats

1.

Department of Gastroenterology, İnönü University School of Medicine, Malatya, Turkey

2.

Department of Biochemistry, İnönü University School of Medicine, Malatya, Turkey

3.

Department of Gastroenterology, Elazığ Training and Research Hospital, Elazığ, Turkey

4.

Department of Microbiology, İnönü University School of Medicine, Malatya, Turkey

5.

Department of Pathology, İnönü University School of Medicine, Malatya, Turkey

6.

Department of Family Medicine, İnönü University School of Medicine, Malatya, Turkey

Turk J Gastroenterol 2017; 28: 394-400
DOI: 10.5152/tjg.2017.1775
Read: 1930 Downloads: 757 Published: 25 July 2019

Abstract

Background/Aims: Steroids have been shown to prevent intestinal oxidative stress. We investigated the effects of methylprednisolone on intestinal oxidative damage and bacterial translocation in thioacetamide-induced liver failure in rats.

 

Materials and Methods: Group 1 (n=8) was the control group. In group 2 (n=8), the thioacetamide group, rats received 300 mg/kg intraperitoneal thioacetamide daily for 2 days. In group 3 (n=8), the thioacetamide+methylprednisolone group, treatment with methylprednisolone (30 mg/kg intraperitoneal) was commenced 48 h before the first dose of thioacetamide. In group 4 (n=8), the methylprednisolone group, the rats received only methylprednisolone (30 mg/kg intraperitoneal).

 

Results: Serious hepatic and intestinal oxidative damage and high bacterial translocation frequencies were observed in the thioacetamide group compared with those of the controls. Bacterial translocation frequency in the thioacetamide+methylprednisolone group was significantly lower than that in the thioacetamide group (p<0.05). Intestinal thiobarbituric acid-reactive substances and myeloperoxidase levels and tissue damage scores for the intestines in the thioacetamide+methylprednisolone group were lower than those in the thioacetamide group (p<0.01, p<0.01, and p<0.0001, respectively).

 

Conclusion: Our findings suggest that methylprednisolone reduces bacterial translocation by preventing intestinal oxidative damage in this model of acute liver failure in rats.

 

 

Cite this article as: Harputluoğlu MMM, Temel İ, Demirel U, et al. Methylprednisolone prevents bacterial translocation in thioacetamide-induced liver failure in rats. Turk J Gastroenterol 2017; 28: 394-400.

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