Turkish Journal of Gastroenterology
Original Article

Matrine Protects Intestinal Barrier Function via MicroRNA-155 Through ROCK1-Signaling Pathway

1.

Department of AnoRectal Surgery, The Tenth Affiliated Hospital of Southern Medical University (Dongguan People’s Hospital), Dongguan, Guangdong, China

2.

Key Laboratory of Biological Targeting Diagnosis, Therapy and Rehabilitation of Guangdong Higher Education Institutes, The Fifth Affiliated Hospital of Guangzhou Medical University, Guangzhou, Guangdong, China

3.

Research Center of Gastroenterology and Hepatology Craiova, University of Medicine and Pharmacy Craiova, Romania

Turk J Gastroenterol 2023; 34: 831-838
DOI: 10.5152/tjg.2023.21884
Read: 1106 Downloads: 344 Published: 01 August 2023

Background: The aim of this study was to investigate the protective effects on intestinal barrier function and tight junction by matrine via a small, non-coding RNA microRNA-155-signaling pathway.

Materials and Methods: Tight junction protein and target gene expressions were determined through microRNA-155 inhibition or overexpression of Caco-2 cell line with or without the treatment of matrine. In order to further verify the role of matrine, dextran sulfate sodium-induced mice colitis was treated by matrine. MicroRNA-155 and ROCK1 expressions were detected in clinical specimens of acute obstruction patients.

Results: Matrine could enhance the expression level of occludin, which might be inhibited by the overexpression of microRNA-155. After the transfection of the precursor of microRNA-155 into Caco-2 cells, ROCK1 expression was increased at both mRNA and protein levels. MicroRNA-155 inhibitor could decrease the ROCK1 expression after transfection. Furthermore, matrine could increase the permeability and decrease tight junction-associated proteins induced by dextran sulfate sodium-induced mice colitis. Clinical sample detection also found high levels of microRNA-155 in stercoral obstruction patients.

Conclusions: Matrine could maintain the tight junction and protect the intestinal barrier from dysfunction. The molecular mechanism may be that matrine might inhibit microRNA-155 and increase the expression level of tight junction proteins.

Cite this article as: Yu D, Su D, Liu Z. Matrine protects intestinal barrier function via MicroRNA-155 through ROCK1-signaling pathway. Turk J Gastroenterol. 2023;34(8):831-838.

Files
EISSN 2148-5607