Turkish Journal of Gastroenterology
Original Article

Influences of proton pump inhibitor on Helicobacter pylori adherence to the gastrointestinal cell lines

1.

Faculty of Pharmacy, University Kebangsaan Malaysia, Kuala Lumpur, Malaysia

Turk J Gastroenterol 2017; 28: 53-59
DOI: 10.5152/tjg.2016.0409
Read: 1623 Downloads: 641 Published: 25 July 2019

Abstract

Background/Aims: Helicobacter pylori is a carcinogenic bacterium that could induce P-glycoprotein expression in the human gastrointestinal tract. Bacterial adherence to the gastrointestinal cell lines could be influenced by the level of P-glycoprotein. This study aimed to determine the influence of proton pump inhibitors that exhibit an inhibitory effect on P-glycoprotein in gastrointestinal carcinoma cell lines, namely Caco-2 and LS174T, in relation to H. pylori adherence.

 

Materials and Methods: Caco-2 and LS174T cells lines treated with omeprazole and esomeprazole were used in this study to assess the bacterial attachment of H. pylori within certain incubation periods.

 

Results: The presence of proton pump inhibitors increased the H. pylori adherence in a time-dependent manner in both Caco-2 and LS174T cell lines. The double inhibition of P-glycoprotein using proton pump inhibitor and P-glycoprotein inhibitor caused low P-glycoprotein expression in the cell lines, resulting in higher H. pylori adherence compared to the control cell lines.

 

Conclusion: Proton pump inhibitors may alter P-glycoprotein expression in the gastrointestinal tract, and subsequently H. pylori adherence on the cell lines, and may contribute to resistance to drug therapy.

Files
EISSN 2148-5607