Original Article

The Investigation of Therapeutic Implications of Mast Cell Stabilizer Cromolyn Sodium on Cholestasis and Cholestatic Pruritus in Experimental Cholestasis

Volume 34 · Issue 1 Publish Date: September 12, 2022
Hanife Yurdakul Ertan Günnur Özbakış Dengiz Figen Barut Yonca Yılmaz Ürün Murat Can Shemsu Umer Hussen Yücel Üstündağ
Full Text PDF
DOI

Published In

Turkish Journal of Gastroenterology

Volume 34 · Issue 1

Pages: 62-72

History

Published Online: September 12, 2022

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Copyright © 2022 Hanife Yurdakul Ertan, Günnur Özbakış Dengiz, Figen Barut, Yonca Yılmaz Ürün, Murat Can, Shemsu Umer Hussen, Yücel Üstündağ

Hanife Yurdakul Ertan
Department of Internal Medicine Bülent Ecevit University Faculty of Medicine Zonguldak Turkey
Günnur Özbakış Dengiz
Department of Medical Pharmacology Zonguldak Bülent Ecevit University Faculty of Medicine Zonguldak Turkey
Figen Barut
Department of Pathology Zonguldak Bülent Ecevit University Faculty of Medicine Zonguldak Turkey
Yonca Yılmaz Ürün
Department of Gastroenterology and Hepatology Eskişehir City Hospital Turkey
Murat Can
Department of Medical Biochemistry Zonguldak Bülent Ecevit University Faculty of Medicine Zonguldak Turkey
Shemsu Umer Hussen
Department of Medical Pharmacology Zonguldak Bülent Ecevit University Faculty of Medicine Zonguldak Turkey
Yücel Üstündağ
Department of Gastroenterology and Hepatology Zonguldak Bülent Ecevit University Faculty of Medicine Zonguldak Turkey
Yurdakul Ertan, H., Özbakış Dengiz, G., Barut, F., Yılmaz Ürün, Y., Can, M., Umer Hussen, S., & Üstündağ, Y. (2022). The Investigation of Therapeutic Implications of Mast Cell Stabilizer Cromolyn Sodium on Cholestasis and Cholestatic Pruritus in Experimental Cholestasis. Turkish Journal of Gastroenterology, 34(1), 62–72. https://doi.org/10.5152/tjg.2022.21744
Endnote/Zotero/Mendeley (RIS) BibTeX
Full Text PDF

Abstract

Background: Relevant studies have indicated that hepatic mast cells may have potential roles in the progression of cholestasis and cholestasis-induced itch. We aimed to compare the effects of cromolyn sodium and other medications on cholestatic pruritus, serum biochemistry, histamine, total bile acids, autotaxin, liver histopathology, and mast cell distribution in tissues in an experimental cholestasis model conducted by bile duct ligation. Methods: Rats received the determined treatment consecutively for 10 days in addition to bile duct ligation. On the 5th and 10th days of the experiment, the rats’ itching behaviors were observed for 5 minutes. After 10 days, blood and tissue samples were taken. Results: Significant decreases in serum histamine and autotaxin levels, plasma total bile acids, total bilirubin, and biliary enzymes were reported only in cromolyn sodium-treated rats compared to the control group. In immunohistochemistry of the liver samples, the peribiliary mast cells stained positive for autotaxin. Except for bile duct infarctus, all histopathological findings of cholestasis significantly improved only in cromolyn sodium-treated and sertraline-treated rats. The liver and peritoneal mast cells significantly decreased only in cromolyn sodium-treated rats compared to the control group. On the 10th day of the experiment, the mean duration of itching was significantly lower in all groups, except for naloxone- and ondansetron-treated rats. Conclusion: Cromolyn sodium has promising antipruritic efficacy and provides biochemical and histopathological recovery of the relevant parameters of cholestasis induced by bile duct ligation. For the first time in the literature, we showed that peribiliary mast cells can produce autotaxin, which is a very important pruritogenic signal in the setting of cholestasis. Cite this article as: Yurdakul Ertan H, Özbakış Dengiz G, Barut F, et al. The investigation of therapeutic implications of mast cell stabilizer cromolyn sodium on cholestasis and cholestatic pruritus in experimental cholestasis. Turk J Gastroenterol. 2023; 34(1): 62-72.

Most read articles by the same author(s)

1 2 3 > >> 
Article Info
Published In
Journal Turkish Journal of Gastroenterology
Volume / Issue Volume 34 · Issue 1
Pages 62-72
DOI 10.5152/tjg.2022.21744
History
Published Online September 12, 2022
License
Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Copyright
Copyright (c) 2022 Hanife Yurdakul Ertan, Günnur Özbakış Dengiz, Figen Barut, Yonca Yılmaz Ürün, Murat Can, Shemsu Umer Hussen, Yücel Üstündağ
Affiliations
Hanife Yurdakul Ertan
Department of Internal Medicine Bülent Ecevit University Faculty of Medicine Zonguldak Turkey
Günnur Özbakış Dengiz
Department of Medical Pharmacology Zonguldak Bülent Ecevit University Faculty of Medicine Zonguldak Turkey
Figen Barut
Department of Pathology Zonguldak Bülent Ecevit University Faculty of Medicine Zonguldak Turkey
Yonca Yılmaz Ürün
Department of Gastroenterology and Hepatology Eskişehir City Hospital Turkey
Murat Can
Department of Medical Biochemistry Zonguldak Bülent Ecevit University Faculty of Medicine Zonguldak Turkey
Shemsu Umer Hussen
Department of Medical Pharmacology Zonguldak Bülent Ecevit University Faculty of Medicine Zonguldak Turkey
Yücel Üstündağ
Department of Gastroenterology and Hepatology Zonguldak Bülent Ecevit University Faculty of Medicine Zonguldak Turkey
Cite this Article
Yurdakul Ertan, H., Özbakış Dengiz, G., Barut, F., Yılmaz Ürün, Y., Can, M., Umer Hussen, S., & Üstündağ, Y. (2022). The Investigation of Therapeutic Implications of Mast Cell Stabilizer Cromolyn Sodium on Cholestasis and Cholestatic Pruritus in Experimental Cholestasis. Turkish Journal of Gastroenterology, 34(1), 62–72. https://doi.org/10.5152/tjg.2022.21744
Download Citation
Endnote/Zotero/Mendeley (RIS) BibTeX
Outlines