Published In
Turkish Journal of Gastroenterology
Volume 33 · Issue 8
Pages: 710-719
History
Published Online: August 15, 2022
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright © 2022 Shen Xiao, Sun Jing, Sun Jiakui, Zou Lei, Liu Ying, Liu Han, Mu Xinwei, Li Weiqin
Shen Xiao
Department of Critical Care Medicine Nanjing First Hospital Nanjing Medical University Nanjing People?s Republic of China
Sun Jing
Department of Gastroenterology, Wuxi People?s Hospital Affiliated to Nanjing Medical University, Wuxi, People?s Republic of China
Sun Jiakui
Department of Critical Care Medicine, Nanjing First Hospital, Nanjing Medical University, Nanjing, People?s Republic of China
Zou Lei
Department of Critical Care Medicine, Nanjing First Hospital, Nanjing Medical University, Nanjing, People?s Republic of China
Liu Ying
Department of Critical Care Medicine, Nanjing First Hospital, Nanjing Medical University, Nanjing, People?s Republic of China
Liu Han
Department of Critical Care Medicine, Nanjing First Hospital, Nanjing Medical University, Nanjing, People?s Republic of China
Mu Xinwei
Department of Critical Care Medicine, Nanjing First Hospital, Nanjing Medical University, Nanjing, People?s Republic of China
Li Weiqin
Department of General Surgery, Surgical Intensive Care Unit (SICU), Jinling Hospital, Medical School of Nanjing University, Nanjing, People?s Republic of China
Xiao, S., Jing, S., Jiakui, S., Lei, Z., Ying, L., Han, L., … Weiqin, L. (2022). Butyrate Ameliorates Intestinal Epithelial Barrier Injury Via Enhancing Foxp3+ Regulatory T-Cell Function in Severe Acute Pancreatitis Model. Turkish Journal of Gastroenterology, 33(8), 710–719. https://doi.org/10.5152/tjg.2022.21307
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Abstract
Background: This study aimed to examine the effect of sodium butyrate on severe acute pancreatitis-related gut barrier injury in a rat model and explore its mechanism. Methods: Male rats randomly fell into 3 groups, that is, the control, the severe acute pancreatitis group, and the severe acute pancreatitis+butyrate group. Rats in the control group received sham operation, while rats in the severe acute pancreatitis group and severe acute pancreatitis+butyrate group received severe acute pancreatitis induction by intraductal infusion of 4% sodium taurocholate. After that, rats in the severe acute pancreatitis+butyrate group were fed with sodium butyrate solution with free access. Intestinal barrier injury was measured based on the expression of tight junction proteins by reverse transcription polymerase chain reaction, Western blotting assay as well as immunohistochemical staining. The variation of Treg cells was measured by reverse transcription polymerase chain reaction, Western blotting assay, immunohistochemical staining, and flow cytometry analysis. Results: Compared to rats in the control, rats in the severe acute pancreatitis group showed significantly higher pathohistological scores (P < .001) in the intestine, as well as decreased expression of occludin and ZO-1. While, rats in the severe acute pancreatitis+butyrate group showed mitigated histologic lesions (P < .05) and increased expressions of occludin and ZO-1. In addition, rats in the severe acute pancreatitis group showed the obvious reduction in the expressions of Foxp3 and GPR109a and the decreased percentage of Treg cells in the intestine (P < .001) compared to rats in the control. However, rats in the severe acute pancreatitis+butyrate group showed markedly increased expressions of Foxp3 and GPR109a and the upregulated percentage of Treg cells (P < .01). Conclusion: Butyrate could significantly mitigate the intestinal injury induced by severe acute pancreatitis, probably by inducing the differentiation of Treg cells. Cite this article as: Xiao S, Jing S, Jiakui S, et al. Butyrate ameliorates intestinal epithelial barrier injury via enhancing Foxp3+ regulatory T-cell function in severe acute pancreatitis model. Turk J Gastroenterol. 2022;33(8):710-719.
Article Info
Published In
Journal
Turkish Journal of Gastroenterology
Volume / Issue
Volume 33 · Issue 8
Pages
710-719
History
Published Online
August 15, 2022
License
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright
Copyright (c) 2022 Shen Xiao, Sun Jing, Sun Jiakui, Zou Lei, Liu Ying, Liu Han, Mu Xinwei, Li Weiqin
Affiliations
Shen Xiao
Department of Critical Care Medicine Nanjing First Hospital Nanjing Medical University Nanjing People?s Republic of China
Sun Jing
Department of Gastroenterology, Wuxi People?s Hospital Affiliated to Nanjing Medical University, Wuxi, People?s Republic of China
Sun Jiakui
Department of Critical Care Medicine, Nanjing First Hospital, Nanjing Medical University, Nanjing, People?s Republic of China
Zou Lei
Department of Critical Care Medicine, Nanjing First Hospital, Nanjing Medical University, Nanjing, People?s Republic of China
Liu Ying
Department of Critical Care Medicine, Nanjing First Hospital, Nanjing Medical University, Nanjing, People?s Republic of China
Liu Han
Department of Critical Care Medicine, Nanjing First Hospital, Nanjing Medical University, Nanjing, People?s Republic of China
Mu Xinwei
Department of Critical Care Medicine, Nanjing First Hospital, Nanjing Medical University, Nanjing, People?s Republic of China
Li Weiqin
Department of General Surgery, Surgical Intensive Care Unit (SICU), Jinling Hospital, Medical School of Nanjing University, Nanjing, People?s Republic of China
Cite this Article
Xiao, S., Jing, S., Jiakui, S., Lei, Z., Ying, L., Han, L., … Weiqin, L. (2022). Butyrate Ameliorates Intestinal Epithelial Barrier Injury Via Enhancing Foxp3+ Regulatory T-Cell Function in Severe Acute Pancreatitis Model. Turkish Journal of Gastroenterology, 33(8), 710–719. https://doi.org/10.5152/tjg.2022.21307
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