Original Article

Role of oxidative stress and insulin resistance in disease severity of non-alcoholic fatty liver disease

Volume 27 · Issue 4 Publish Date: July 15, 2016
Emine Köroğlu Billur Canbakan Kadri Atay İbrahim Hatemi Murat Tuncer Ahmet Dobrucalı Abdullah Sonsuz İlhami Gültepe Hakan Şentürk
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Published In

Turkish Journal of Gastroenterology

Volume 27 · Issue 4

Pages: 361-366

History

Published Online: July 15, 2016

Copyright © 2016 Emine Köroğlu, Billur Canbakan, Kadri Atay, İbrahim Hatemi, Murat Tuncer, Ahmet Dobrucalı, Abdullah Sonsuz, İlhami Gültepe, Hakan Şentürk

Emine Köroğlu
Department of Gastroenterology, Kartal Dr. Lütfi Kırdar Training and Research Hospital, İstanbul, Turkey
Billur Canbakan
Department of Gastroenterology, İstanbul University Cerrahpaşa School of Medicine, İstanbul, Turkey
Kadri Atay
Department of Gastroenterology, İstanbul University Cerrahpaşa School of Medicine, İstanbul, Turkey
İbrahim Hatemi
Departments of Gastroenterology, İstanbul University Cerrahpaşa Medical Faculty, İstanbul
Murat Tuncer
Department of Gastroenterology, İstanbul University Cerrahpaşa School of Medicine, İstanbul, Turkey
Ahmet Dobrucalı
Department of Gastroenterology, İstanbul University Cerrahpaşa School of Medicine, İstanbul, Turkey
Abdullah Sonsuz
Cerrahpaşa Tıp Fakültesi İç Hastalıkları Anabilim Dalları, İstanbul
İlhami Gültepe
Department of Internal Medicine, Bezmialem Vakıf University Hospital, İstanbul, Turkey
Hakan Şentürk
Department of Gastroenterology, Bezmialem Vakıf University Faculty of Medicine, İstanbul, Turkey
Köroğlu, E., Canbakan, B., Atay, K., Hatemi, İbrahim, Tuncer, M., Dobrucalı, A., … Şentürk, H. (2016). Role of oxidative stress and insulin resistance in disease severity of non-alcoholic fatty liver disease. Turkish Journal of Gastroenterology, 27(4), 361–366. https://doi.org/10.5152/tjg.2016.16106
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Abstract

Abstract Background/Aims: Oxidative stress and insulin resistance (IR) are major contributors in the pathogenesis of non-alcoholic fatty liver disease (NAFLD). The purpose of this study was to find the relation between oxidative stress parameters and histopathological findings in NAFLD patients with and without insulin resistance (IR). Materials and Methods: Thirty-two patients with no alcohol intake and biopsy-proven diagnosis of NAFLD were studied (M/F: 17/15; mean age 46.5±11.4 years). Twenty-one NAFLD patients with IR were compared with 11 patients without IR. The fasting insulin level was measured, and the insulin resistance index was calculated using the homeostasis model assessment (HOMA) method. Malondialdehyde (MDA) and superoxide dismutase (SOD) activities were measured in tissue and serum specimens. Glutathione (GH) was measured in tissue homogenates. Nitric oxide (NO), vitamin E and C levels were measured in serum. Results: Patients with IR had significantly higher tissue MDA levels (p=0.001) and significantly decreased tissue SOD and GH levels (p=0.001 and 0.002, respectively) than those without IR. The steatosis grade, necroinflammatory grade and stage were significantly higher in patients with IR (p=0.035, 0.003 and 0.001, respectively). HOMA IR significantly correlated with the necroinflammatory grade, stage, tissue MDA, SOD and GH (p=0.013, 0.001, 0.008, 0.001 and 0.001, respectively). Serum MDA (β=1.88, p=0.002), serum SOD (β=0.57, p=0.006), tissue MDA (β=0.22, p=0.006), tissue SOD (β=1.48, p=0.071) and stage (β=2.81, p=0.003) were independently associated with increased HOMA IR. Increased MDA [OR: 1.51; 95% CI: (1.03–2.22); p=0.034] was a risk factor for non-alcoholic steatohepatitis (NASH), and increased SOD activity had a preventive effect against NASH [OR: 0.008; 95% CI: (0.001–0.98); p=0.04]. Conclusion: This study shows that insulin resistance in NAFLD correlates with enhanced oxidative stress. Histopathological disease severity significantly correlated with oxidative stress parameters. These data show that NAFLD patients with IR may have increased risk for disease progression.

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Article Info
Published In
Journal Turkish Journal of Gastroenterology
Volume / Issue Volume 27 · Issue 4
Pages 361-366
DOI 10.5152/tjg.2016.16106
History
Published Online July 15, 2016
Copyright
Copyright (c) 2016 Emine Köroğlu, Billur Canbakan, Kadri Atay, İbrahim Hatemi, Murat Tuncer, Ahmet Dobrucalı, Abdullah Sonsuz, İlhami Gültepe, Hakan Şentürk
Affiliations
Emine Köroğlu
Department of Gastroenterology, Kartal Dr. Lütfi Kırdar Training and Research Hospital, İstanbul, Turkey
Billur Canbakan
Department of Gastroenterology, İstanbul University Cerrahpaşa School of Medicine, İstanbul, Turkey
Kadri Atay
Department of Gastroenterology, İstanbul University Cerrahpaşa School of Medicine, İstanbul, Turkey
İbrahim Hatemi
Departments of Gastroenterology, İstanbul University Cerrahpaşa Medical Faculty, İstanbul
Murat Tuncer
Department of Gastroenterology, İstanbul University Cerrahpaşa School of Medicine, İstanbul, Turkey
Ahmet Dobrucalı
Department of Gastroenterology, İstanbul University Cerrahpaşa School of Medicine, İstanbul, Turkey
Abdullah Sonsuz
Cerrahpaşa Tıp Fakültesi İç Hastalıkları Anabilim Dalları, İstanbul
İlhami Gültepe
Department of Internal Medicine, Bezmialem Vakıf University Hospital, İstanbul, Turkey
Hakan Şentürk
Department of Gastroenterology, Bezmialem Vakıf University Faculty of Medicine, İstanbul, Turkey
Cite this Article
Köroğlu, E., Canbakan, B., Atay, K., Hatemi, İbrahim, Tuncer, M., Dobrucalı, A., … Şentürk, H. (2016). Role of oxidative stress and insulin resistance in disease severity of non-alcoholic fatty liver disease. Turkish Journal of Gastroenterology, 27(4), 361–366. https://doi.org/10.5152/tjg.2016.16106
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