E-ISSN 2148-5607
Original Article
Role of Th1/Th2 cells and related cytokines in autoimmune hepatitis
1 Clinical Genetics Department, National Institute of Genetic Engineering and Biotechnology Tehran, Iran  
2 Digestive Diseases Research Center, Digestive Diseases Research Institute, Tehran University of Medical Sciences, Tehran, Iran  
3 School of Public Health, Tehran University of Medical Sciences, Tehran, Iran  
Turk J Gastroenterol 2017; 28: 110-114
DOI: 10.5152/tjg.2017.17501
Key Words: Autoimmune hepatitis, gene expression, cytokines

Background/Aims: Dysregulation of T cell response is thought to play an important role in the immunopathogenesis of autoimmune hepatitis. However, no consensus has yet been reached regarding the implications of a distinct T cell subset in the pathogenesis of this progressive liver disease. Therefore, T-bet and GATA-3 expression was examined in patients with autoimmune hepatitis (AIH) and in healthy controls. Moreover, the profile of Th1 (IFN-γ) and Th2 (IL-4) cytokine gene expression was analyzed.


Materials and Methods: Levels of mRNA transcripts were measured in peripheral blood mononuclear cells (PBMCs) using a two-step reverse transcription quantitative real-time polymerase chain reaction with SYBR Green.


Results: T-bet and IFN-γ mRNA expression was significantly higher in AIH patients compared to healthy controls (p<0.05), whereas no differences were observed for either GATA-3 or IL-4 mRNA expression (p>0.05).


Conclusion: Alterations in the Th1/Th2 cell balance may be responsible for both disease progression and the resulting complications.

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