ISSN 1300-4948 | E-ISSN 2148-5607
Gastrointestinal Tract - Original Article
Potential role of chromatin remodeling factor genes in atrophic gastritis/gastric cancer risk
1 Department of Internal Medicine, Celal Bayar University School of Medicine, Manisa, Turkey  
2 Department of Gastroenterology, Gazi Hospital, İzmir, Turkey  
3 Department of Medical Genetics, Adnan Menderes University School of Medicine, Aydın, Turkey  
4 Department of Gastroenterology, Celal Bayar University School of Medicine, Manisa, Turkey  
5 Department of Medical Biology, Celal Bayar University School of Medicine, Manisa, Turkey  
6 Department of Gastroenterology, Erzurum Regional Training and Research Hospital, Erzurum, Turkey  
Turk J Gastroenterol 2018; 29: 427-435
DOI: 10.5152/tjg.2018.17350
Key Words: Keywords: Atrophic gastritis, chromatin remodeling, epigenetic, gastric cancer, Helicobacter pylori, intestinal metaplasia
Abstract

 

Background/Aims: Atrophic gastritis (AG), intestinal metaplasia (IM), and Helicobacter pylori (HP) are the risk factors for the development of gastric cancer (GC). Chromatin remodeling is one of the epigenetic mechanisms involved in the carcinogenesis of GC. The purpose of this study was to investigate the expression profiles of defined chromatin remodeling genes in gastric mucosal samples and their values as gastric carcinogenesis biomarkers.

 

Materials and Methods: In total, 95 patients were included in the study. Patients were divided into 3 groups as: GC group (n=34), AG group (n=36), and control group (n=25). AG group was further divided into subgroups based on the presence of HP and IM in gastric mucosa. Chromatin remodeling gene expressions were analyzed using real-time PCR (RT-PCR) array in all groups. Data were evaluated using the RT-qPCR primer assay data analysis software.

 

Results: EED, CBX3, and MTA1 were more overexpressed, whereas ARID1A, ING5, and CBX7 were more underexpressed in the AG and GC groups compared with the controls. No significant differences were observed between the AG and GC groups concerning the expression of these 6 genes, although the fold change levels of these genes in the GC group were well above than in the AG group. EED, CBX3, and MTA1 were significantly more overexpressed in HP- and IM-positive AG subgroup compared with the HP- or IM-negative AG subgroup.

 

Conclusion: In conclusion, our results provide an evidence of epigenetic alterations in AG. Expressions of EED, CBX3, MTA1, ARID1A, ING5, and CBX7 may be considered as promising markers to be used in GC screening for patients with AG.

 

Cite this article as: Bilgiç F, Gerçeker E, Örenay Boyacıoğlu S, et al. Potential role of chromatin remodeling factor genes in atrophic gastritis/gastric cancer risk. Turk J Gastroenterol 2018; 29: 427-35.

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AVES | Copyright © 2018 Turkish Society of Gastroenterology | Latest Update: 07.09.2018