ISSN 1300-4948 | E-ISSN 2148-5607
Original Article
Association of a single nucleotide polymorphism in the TLR2 gene (rs3804099) but not in the TLR4 gene (rs4986790) with Helicobacter pylori infection and peptic ulcer
1 Medical Biotechnology Research Center, Islamic Azad University, Yazd, Iran  
2 Department of Immunology, Kerman University of Medical Sciences, School of Medicine, Kerman, Iran; Department of Laboratory, Kerman University of Medical Sciences, Sciences, School of Para-Medicine, Kerman University of Medical Sciences, Kerman, Iran.  
3 Gastroenterology and Hepatology Research Center, Kerman University of Medical Sciences, Institute of Basic and Clinical Physiology Sciences, Kerman, Iran  
4 Department of Immunology, Kerman University of Medical Sciences, School of Medicine,, Kerman,, Iran  
5 Department of Immunology, Kerman University of Medical Sciences, School of Medicine, Kerman,, Iran; Immunology of Infectious Diseases Research Center, Rafsanjan University of Medical Sciences, Rafsanjan, Iran.  
Turk J Gastroenterol ; : -
DOI: 10.5152/tjg.2018.17484
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Key Words: Helicobacter pylori, peptic ulcer, TLR2, TLR4, gene polymorphism
Abstract

Background/Aims: Toll like receptors (TLRs), particularly TLR2 and TLR4 take part in elicitation of immune responses against H. pylori. This study aimed to investigate the relationship between single nucleotide polymorphisms (SNP) rs3804099 in TLR2 gene and rs4986790 in TLR4 gene with H. pylori infection and peptic ulcer (PU).

 

Materials and Methods: Blood specimens were obtained from 350 individuals including 100 H. pylori-infected patients with PU, 125 H. pylori-infected asymptomatic subjects (AS) and 125 non-infected healthy subjects (NHS). The DNA was extracted and the SNPs determined using ARMS-PCR method.

 

Results: The frequency of CT genotype at TLR2 SNP rs3804099 in both PU and AS groups was significantly higher than NHS group (P<0.05). In total H. pylori-infected individuals (PU+AS), the frequency of CT genotype at rs3804099 was also significantly higher than NHS group (P<0.005). The frequency CC genotype at rs3804099 in total H. pylori-infected individuals (PU+AS) was markedly lower than NHS group (P=0.066). PU patients carried higher frequency of CT genotype than total healthy individuals (AS+NHS) (P<0.03). The distribution of TT genotype was lower whereas the frequency of CT genotype was higher in AS individuals infected with CagA+ strains than those infected with CagA- strains (P<0.03). No significant differences found between PU, AS and NHS groups regarding the genetic differences at rs4986790 in TLR4 gene.

 

Conclusion: These results provide evidences regarding the association of the rs3804099 in TLR2 gene with H. pylori infection and PU. The rs3804099 may affect vulnerability to H. pylori infection, particularly to CagA+ strains of bacteria. 

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AVES | Copyright © 2018 Turkish Society of Gastroenterology | Latest Update: 30.01.2018