ISSN 1300-4948 | E-ISSN 2148-5607
Basic & Translational - Original Article
Methylprednisolone prevents bacterial translocation in thioacetamide-induced liver failure in rats
1 Department of Gastroenterology, İnönü University School of Medicine, Malatya, Turkey  
2 Department of Biochemistry, İnönü University School of Medicine, Malatya, Turkey  
3 Department of Gastroenterology, Elazığ Training and Research Hospital, Elazığ, Turkey  
4 Department of Microbiology, İnönü University School of Medicine, Malatya, Turkey  
5 Department of Pathology, İnönü University School of Medicine, Malatya, Turkey  
6 Department of Family Medicine, İnönü University School of Medicine, Malatya, Turkey  
Turk J Gastroenterol 2017; 28: 394-400
DOI: 10.5152/tjg.2017.1775
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Key Words: Methylprednisolone, liver failure, bacterial translocation
Abstract

Background/Aims: Steroids have been shown to prevent intestinal oxidative stress. We investigated the effects of methylprednisolone on intestinal oxidative damage and bacterial translocation in thioacetamide-induced liver failure in rats.

 

Materials and Methods: Group 1 (n=8) was the control group. In group 2 (n=8), the thioacetamide group, rats received 300 mg/kg intraperitoneal thioacetamide daily for 2 days. In group 3 (n=8), the thioacetamide+methylprednisolone group, treatment with methylprednisolone (30 mg/kg intraperitoneal) was commenced 48 h before the first dose of thioacetamide. In group 4 (n=8), the methylprednisolone group, the rats received only methylprednisolone (30 mg/kg intraperitoneal).

 

Results: Serious hepatic and intestinal oxidative damage and high bacterial translocation frequencies were observed in the thioacetamide group compared with those of the controls. Bacterial translocation frequency in the thioacetamide+methylprednisolone group was significantly lower than that in the thioacetamide group (p<0.05). Intestinal thiobarbituric acid-reactive substances and myeloperoxidase levels and tissue damage scores for the intestines in the thioacetamide+methylprednisolone group were lower than those in the thioacetamide group (p<0.01, p<0.01, and p<0.0001, respectively).

 

Conclusion: Our findings suggest that methylprednisolone reduces bacterial translocation by preventing intestinal oxidative damage in this model of acute liver failure in rats.

 

 

Cite this article as: Harputluoğlu MMM, Temel İ, Demirel U, et al. Methylprednisolone prevents bacterial translocation in thioacetamide-induced liver failure in rats. Turk J Gastroenterol 2017; 28: 394-400.

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